Gibco™ Mouse NGF 7S Subunit Native Protein

Description

Native Protein

Reconstitution: Reconstitute murine natural NGF 7S to a stock concentration of 100 μg/mL with 1 mL of sterile culture medium containing 1-2% (w/v) BSA. Aliquot into single-use portions in polypropylene vials and store at -20°C to avoid repeated freeze/thaw cycles. Do not store in dilute solution. Dilute immediately before use. To avoid loss of protein or activity, prepare dilute solutions in appropriate buffer or medium containing at least 0.1% (w/v) BSA or 1-10% (v/v)serum. Carrier protein (BSA or serum) is needed to stabilize the NGF protein.

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Accession Number: P01139
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: potassium phosphate with no preservative
• Gene ID (Entrez): 18049
• Name: Mouse NGF 7 S Subunit
• Quantity: 100 μg
• Gene Alias: B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
• Common Name: NGF
• Biological Activity: ED50 = 5 - 100 ng/mL; determined by the dose-dependent neurite outgrowth of chick embryo dorsal root ganglia.
• Conjugate: Unconjugated
• Species: Mouse
• Content And Storage: -20°C
• Shipping Condition: Dry Ice
• Protein Family: Growth Factors & Receptors
• Protein Form: Native, Ligand
• Form: Lyophilized
• Protein Subtype: Neurotrophic Factors
• Research Category: Neurobiology, Signal Transduction, Oncology, Stem Cell Research, Inflammation, Differentiation, Cardiovascular Research
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

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