Invitrogen™ Mouse NT-proBNP ELISA Kit

Catalog No.	Quantity
EEL193	96 Tests

Catalog No. EEL193 Supplier Invitrogen™ Supplier No. EEL193

Description

ELISA

The Mouse N terminal pro Brain Natriuretic Peptide (NT proBNP) ELISA quantitates NT proBNP in serum, plasma and other biological fluids. Principle of the method The Mouse N terminal pro Brain Natriuretic Peptide (NT proBNP) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The N-terminal prohormone of brain natriuretic peptide is a prohormone with a 76 amino acid N-terminal inactive protein that is cleaved from the molecule to release brain natriuretic peptide 32.

Specifications

• Accession Number: P40753
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: N-terminal pro b-type natriuretic peptide; NTproBNPN-terminal pro b-type natriuretic peptide; NTproBNP;
• Gene ID (Entrez): 18158
• Gene Symbol: nppb
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: NT-proBNP
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min