Gibco™ Mouse PDGF-AA Recombinant Protein, PeproTech®

Catalog No.	Quantity
315-17-10UG	10 μg
31517100UG	100 μg
315-17-1MG	1 mg
31517250UG	250 μg
315-17-2UG	2 μg
31517500UG	500 μg

Catalog No. 315-17-10UG Supplier Gibco™ Supplier No. 3151710UG

Description

Recombinant Protein

Recombinant Murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Specifications

• Accession Number: P20033
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 18590
• Molecular Weight (g/mol): 28.7 kDa
• Name: Mouse PDGF-AA
• Quantity: 10 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: H-PDGF-AA; PDGF A-chain; PDGF subunit A; PDGF1; PDGF-1; Pdgfa; PDGF-A; PDGFACP; platelet derived growth factor subunit A; platelet derived growth factor, alpha; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A; Rpa1
• Common Name: PDGF-AA
• Gene Symbol: Pdgfa
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATSNLNP DHREEETGRR RESGKNRKRK RLKPT
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.