Invitrogen™ Mouse Peptide YY ELISA Kit

Catalog No.	Quantity
EEL196	96 Tests

Catalog No. EEL196 Supplier Invitrogen™ Supplier No. EEL196

Description

ELISA

The Mouse Peptide YY (PYY) ELISA quantitates PYY in serum, plasma and other biological fluids. Principle of the method The Mouse Peptide YY (PYY) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Peptide tyrosine-tyrosine amide (3-36), or PYY (3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation. PYY (3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY (3-36) is additive to that of the co-secreted gut hormone GLP-1.

Specifications

• Accession Number: Q9EPS2
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: GHYY; Peptide tyrosine tyrosine; peptide tyrosine-tyrosine (YY); peptide YY; peptide YY (mapped); Peptide YY(3-36); Peptide YY3-36; Peptide YY3-37; peptide-YY; prepro-PYY; PYY; PYY II; PYY1; PYY-1; PYY-2; PYYI; PYY-I; PYYII; PYY-II; RATGHYY; YyGHYY; Peptide tyrosine tyrosine; peptide tyrosine-tyrosine (YY); peptide YY; peptide YY (mapped); Peptide YY(3-36); Peptide YY3-36; Peptide YY3-37; peptide-YY; prepro-PYY; PYY; PYY II; PYY1; PYY-1; PYY-2; PYYI; PYY-I; PYYII; PYY-II; RATGHYY; Yy;
• Gene ID (Entrez): 217212
• Gene Symbol: Pyy
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: PYY
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min