Invitrogen™ Mouse PF4 ELISA Kit

Catalog No.	Quantity
EEL120	96 Tests

Catalog No. EEL120 Supplier Invitrogen™ Supplier No. EEL120

Description

ELISA

The Mouse Platelet Factor 4 (PF4) ELISA quantitates PF4 in serum, plasma, and other biological fluids The Mouse Platelet Factor 4 (PF4) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

Specifications

• Accession Number: Q9Z126
• Assay Range: 6.25-400 ng/mL
• Assay Sensitivity: 3.75 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Intercrine alpha (chemokine CxC)
• Sample Type: Plasma, Serum
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4;
• Gene ID (Entrez): 56744
• Gene Symbol: Pf4
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: PF4
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min