Invitrogen™ Mouse Pref-1/DLK-1/FA1 ELISA Kit

Description

ELISA

Mouse Pref-1/DLK-1/FA1 quantitates mouse Pref-1/DLK-1/FA1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Pref-1/DLK-1/FA1.

The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.

Specifications

• Accession Number: Adipocyte differentiation inhibitor protein; AW742678; delta like non-canonical Notch ligand 1; Delta1; delta-like 1 homolog; delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; DlkI; FA1; Fetal antigen 1; Ly107; Peg9; pG2; Preadipocyte factor 1; preadipocyte factor-1; PREF; PREF1; Pref-1; Protein delta homolog 1; SCP1; Scp-1; secredeltin; stromal cell derived protein 1; Zog
• Assay Range: 4.5 to 1,000 pg/mL
• Assay Sensitivity: 4.5 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 13386
• Gene Symbol: DLK1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: DLK1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins