Invitrogen™ Mouse Synuclein alpha ELISA Kit

Catalog No.	Quantity
EEL195	96 Tests

Catalog No. EEL195 Supplier Invitrogen™ Supplier No. EEL195

Description

ELISA

The Mouse Synuclein alpha (SNCalpha) ELISA quantitates SNCalpha in serum, plasma and other biological fluids. Principle of the method The Mouse Synuclein Alpha (SNCa) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

Specifications

• Accession Number: O55042
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Alpha synuclein; alphaSYN; alpha-synuclein; NACP; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; Snca; Syn; synuclein alpha; synuclein alpha-140; synuclein, alpha; synuclein, alpha (non A4 component of amyloid precursor)Alpha synuclein; alphaSYN; alpha-synuclein; NACP; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; Snca; Syn; synuclein alpha; synuclein alpha-140; synuclein, alpha; synuclein, alpha (non A4 component of amyloid precursor);
• Gene ID (Entrez): 20617
• Gene Symbol: SNCA
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: alpha Synuclein
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min