Invitrogen™ Mouse TARC (CCL17) ELISA Kit

Catalog No.	Quantity
EMCCL17X5	5 x 96 Tests
EMCCL17	96 Tests
EMCCL17X10	10 x 96 Tests

Catalog No. EMCCL17X5 Supplier Invitrogen™ Supplier No. EMCCL17X5

Description

ELISA

The Mouse TARC/CCL17 ELISA quantitates Ms TARC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TARC. Principle of the method The Mouse TARC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.

Specifications

• Accession Number: A-152E5.3; ABCD-2; CC chemokine TARC; C-C motif chemokine; C-C motif chemokine 17; C-C motif chemokine ligand 17; CCL17; chemokine (C-C motif) ligand 17; Scya17; Scya17l; small inducible cytokine subfamily A (Cys-Cys), member 17; small inducible cytokine subfamily A17; small-inducible cytokine A17; T cell-directed CC chemokine; TARC; Thymus and activation-regulated chemokine
• Assay Range: 4.12-3000 pg/mL
• Assay Sensitivity: 4 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: A-152E5.3; ABCD-2; CC chemokine TARC; C-C motif chemokine; C-C motif chemokine 17; C-C motif chemokine ligand 17; CCL17; chemokine (C-C motif) ligand 17; SCYA17; Scya17l; small inducible cytokine subfamily A (Cys-Cys), member 17; small inducible cytokine subfamily A17; small-inducible cytokine A17; T cell-directed CC chemokine; TARC; Thymus and activation-regulated chemokineA-152E5.3; ABCD-2; CC chemokine TARC; C-C motif chemokine; C-C motif chemokine 17; C-C motif chemokine ligand 17; CCL17; chemokine (C-C motif) ligand 17; SCYA17; Scya17l; small inducible cytokine subfamily A (Cys-Cys), member 17; small inducible cytokine subfamily A17; small-inducible cytokine A17; T cell-directed CC chemokine; TARC; Thymus and activation-regulated chemokine; Abcd-2; Scya17; Scya17l; Tarc
• Gene ID (Entrez): 20295
• Gene Symbol: CCL17
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: TARC
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min