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Invitrogen™ Th1/Th2 Mouse Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 88771144

Catalog No. 50-174-22

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Contains Azide

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution



The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.


P01580, P04351, P07750, P18893
15978, 16153, 16183, 16189
Assay Diluent: 5X concentrated
Plasma, Serum, Supernatants
1 hr 45 min
IFNg, IL-10, IL-2, IL-4
IFN-gamma: 15-2,000 pg/mL; IL-2: 2-200 pg/mL; IL-4: 4-500 pg/mL; IL-10: 30-4,000 pg/mL
Colorimetric Microplate Reader
IFN-gamma: 15 pg/mL; IL-2: 2 pg/mL; IL-4: 4 pg/mL; IL-10: 30 pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
24 hr 30 min
Ifng, Il10, Il2, Il4
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