Invitrogen™ eBioscience™ Mouse TNF alpha ELISA Ready-SET-Go!™ Kit 

Engineered for accurate and precise measurement of mouse TNF alpha protein levels from samples including serum, plasma, tissue homogenate, lavage fluid and cell culture supernatants. Recognition of mouse TNF alpha is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.

Manufacturer:  Invitrogen™ 88732422

Catalog No. 50-112-8954

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.

Specifications

• Accession Number: P06804
• Conjugate: Biotin
• Gene ID (Entrez): 21926
• Kit Contents: 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
• Quantity: 2 x 96 tests
• Sensitivity: 8 pg/mL
• Sample Volume: Lavage Fluid,100 μL|Plasma,100 μL|Serum,100 μL|Supernatant,100 μL|Tissue Homogenate,100 μL
• Storage Requirements: 2-8°C
• Total Assay Time: 24 hr 30 min
• Gene Symbols: Tnf
• Label or Dye: HRP

• Assay Range: 8-1,000 pg/mL
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Type: Lavage Fluid,Plasma,Serum,Supernatant,Tissue Homogenate
• Species: Mouse
• Test Time: 1 hr 45 min
• Target: Mouse
• Synonym: Tumor Necrosis Factor alpha, tnf-a,tnf alpha

For Research Use Only