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Invitrogen™ eBioscience™ Mouse TNF-alpha Platinum ELISA Kit Non-distribution product as customer accommodation.

For the quantitative detection of mouse TNF-alpha.

Manufacturer:  Invitrogen™ BMS6073

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Catalog No. 50-112-5453


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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse TNF-alpha
Biotin-Conjugate anti-mouse TNF-alpha polyclonal antibody
Streptavidin-HRP
Mouse TNF-alpha Standard lyophilized, 2ng/mL upon reconstitution
Calibrator Diluent
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
Specifications

Specifications

P06804
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
3 hr 30 min
Tnf
5.7%
HRP
31.3-2,000 pg/mL
21926
Adhesive Plate Covers
96 tests
3.7 pg/mL
50μL
Mouse
1 hr 20 min
Mouse
Cachectin, Tumor necrosis factor alpha, DIF
6.5%
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