Gibco™ Mouse TRAIL (TNFSF10) Recombinant Protein, PeproTech®

Catalog No.	Quantity
315-19-10UG	10 μg
31519100UG	100 μg
315-19-1MG	1 mg
31519250UG	250 μg
31519500UG	500 μg
315-19-50UG	50 μg

Catalog No. 315-19-10UG Supplier Gibco™ Supplier No. 3151910UG

Description

Recombinant Protein

Recombinant Murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF-homologous portion of the extracellular domain of the full length TRAIL protein. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

Specifications

• Accession Number: P50592
• For Use With (Application): Bioactivity, Control, Western Blot Control
• Formulation: Protein with no preservative
• Gene ID (Entrez): 22035
• Molecular Weight (g/mol): 20 kDa
• Name: Mouse TRAIL (TNFSF10)
• Purification Method: Purified
• Quantity: 10 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; Tnfsf10; TNLG6A; TRAIL; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
• Common Name: TRAIL (CD253)
• Gene Symbol: TNFSF10
• Biological Activity: Assay 1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml Assay 2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN
• Content And Storage: -20°C
• Activity: Assay 1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay 2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.