Gibco™ Mouse Wnt-3a Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Murine Wnt-3a is a monomeric glycoprotein containing 334 amino acid residues. Due to glycosylation, the Murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

Specifications

• Accession Number: P27467
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 22416
• Molecular Weight (g/mol): 37.4 kDa
• Name: Mouse Wnt-3 A
• Quantity: 10 μg
• Source: Mammalian Cell
• Regulatory Status: RUO
• Endotoxin Concentration: <2 EU/ μg
• Gene Alias: MGC119418; MGC119419; MGC119420; Protein Wnt-3 A; vestigial tail; vt; wingless-related MMTV integration site 3 A; wingless-type MMTV integration site family member 3 A; wingless-type MMTV integration site family, member 3 like; wingless-type MMTV integration site family, member 3 A; Wnt 3 A; Wnt family member 3 A; wnt[a]; wnt3; wnt3 l; wnt3 like; Wnt3a; Wnt-3 A; WNT3A protein; wnt3l; Zwnt[a]
• Common Name: WNT3A
• Gene Symbol: WNT3A
• Biological Activity: Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 ?for this effect is 0.4-0.6 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK
• Content And Storage: -20°C
• Expression System: Mammalian cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 75% by SDS-PAGE gel and HPLC analyses.