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MP Biomedicals™ FastRNA™ Pro Soil-Direct Kit

Used to isolate total cellular RNA from microorganisms and other specimens found in soil samples. Designed for use with FastPrep™ Instrument. Two levels of RNA purification, for different soil samples and downstream applications.

Manufacturer:  MP Biomedicals™ 116070050

Catalog No. MP116070050


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Includes: RNApro™, Soil Lysis Solution, Phenol:Chloroform (1:1), Inhibitor Removal Solution, RNAMATRIX™, Slurry, Binding Solution, Wash Solution Concentrate, DEPC-Treated Water, Lysing Matrix E 50

Requires : RNase Erase™ recommended, FastPrep™ Instrument or alternative high speed disruptor, 100% Ethanol, Microcentrifuge, Chilled 70% Ethanol (prepared with DEPC-treated H2O), Chilled Isopropanol, LRNase-free Microcentrifuge

Description

Description

  • Used with FastPrep™-24; FastPrep™-96 homogenizers
  • 2mL tube compatibility
    Basic Protocol Outline
  • Mix sample with Lysing Matrix and Lysis Solution
  • Process with FastPrep Instrument
  • Centrifuge and keep liquid
  • Incubate with Phenol:Chloroform, centrifuge, keep upper aqueous phase
  • Add Inhibitor Removal Solution, centrifuge, and keep liquid
  • Perform Isopropanol precipitation
  • Use RNAMATRIX Binding Solution, RNAMATRIX Slurry, RNAMATRIX Wash Solution, and microcentrifuge to produce isolated RNA sample
Specifications

Specifications

Nucleic Acid isolation and purification, Protein isolation, molecular biology, gene expression, microbiological analysis
Soil Lysis solution, (1:1) Phenol : Chloroform, inhibitor removal solution, RNAMATRIX™, slurry, binding solution, wash solution concentrate, DEPC-treated Water, lysing matrix E 50
Lysing matrix E
50 x 2 mL Tubes
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