MP Biomedicals™ Gnome™ DNA Isolation Kit

Description

Gnome™ DNA Isolation Kit offers a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types.

• No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
• The GNOME™ kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary ''salting-out'' technique which precludes the need for phenol, chloroform, or other organic extractions.
• The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE.
• The GNOME™ DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells.
• Each preparation with the GNOME™ kit yields up to 100 μg of genomic DNA.
• The DNA isolated by the GNOME™ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.

Specifications

• Format: Kit
• Sample Type: Blood, Tissues, Yeast, Bacteria, Plant
• Includes: Cell Lysis/Denaturing Solution, RNase Mixx, Protease Mixx, Salt Out Mixture, Cell Suspension Solution
• No. of Reactions: 100 Preps
• pH: Cell Lysis/Denaturing Solution pH range: 6.2 to 6.9, Salt-out Mixture pH range: 6.0 to 6.8, Cell Suspension Solution pH range: 8.0 to 8.2
• Product Type: Gnome™ DNA Isolation Kit
• Sample Volume: 20 to 50 mg wet weight bacterial cell pellet, Up to 250 mg plant tissue, 2 to 5 x 107 nucleated white blood cells, 50 to 250 mg mammalian tissue, ∼1 to 5 x 108 yeast cell pellet
• Yield: 100 μg

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.