MP Biomedicals™ Protease (S. Aureus) V.8

Description

Protease (S. Aureus) V.8, also called endoproteinase Glu-C, Glu-C protease, staphylococcal serine proteinase. Widely used for protein sequencing, this enzyme specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids.

• Protease V.8 specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids
• In the presence of ammonium, the specificity is limited to glutamic sites
• Used in a study to investigate immobilized residue-specific endoproteinases for protein sequencing
• Protease V8 was also used in a study to assess enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation
• Provides:
  • Robust activity, greater than 915 units/mg protein
  • Specific cleavage for glutamic acid in ammonium buffer
  • Selective digestion at both glutamic and aspartic acid residues in phosphate buffer at pH 7.8
  • Cleavage activity remains in the presence of many denaturing agent, such as SDS, urea and guanidine-HCl
  • Lyophilized format for longer stability and easier storage

Specifications

• For Use With (Application): Used in a study to investigate immobilized residue-specific endoproteinases for Protein sequencing
• pH Range: 4 and 7.8
• Sample Type: Staphylococcus aureus
• Content And Storage: 2°C to 8°C
• Form: Lyophilized
• Quantity: 5 mg
• Product Type: Protease

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov