MP Biomedicals™ M9 Minimal Medium Salts (Maniatis Formulation), Powdered

Description

Used for preparing a defined synthetic medium for the growth of E. coli host

• A commonly used base for preparing a defined synthetic medium for the growth of most E. coli host cells
• Powder is reconstituted in sterile purified water, (or water with melted purified agar added)
• Additional nutrients, such as glucose or other carbon source, casamino acids, vitamins, or antibiotics are added when the solution cools
• Add additional nutrients before plates are poured or media is inoculated with bacteria

Specifications

• Certifications/Compliance: Molecular Biology Certified
• Concentration: 56.4 g/L of a 5X Solution
• Content And Storage: 15° to 30°C
• Description: A 56.4 g sample of M9 Minimal Salts was dissolved in an aqueous solution to 1 liter, the pH adjusted to 7.4 (if needed), and the solution autoclaved to make a sterile 5X concentrate.
• Form: Powder
• Media Type: M9 Minimal Medium
• Product Type: Microbiology Media
• Sterility: Sterile
• Formulation: 33.9 g Na₂HPO₄; 15 g KH₂PO₄; 2.5 g NaCl; 5 g NH₄Cl
• For Use With (Application): Preparing a Defined Synthetic Medium for The Growth of E. Coli Host
• pH: 6.8
• Quantity: 227 g
• Shipping Condition: Ambient
• Solubility: Soluble Aqueous Solution
• Specimen Type: Bacterial
• Strain Designations: E. coli