Thermo Scientific™ Mr. Frosty™ Freezing Container

Catalog No.	Holds
15-350-50A	12 Tubes (3.6 mL)
15-350-50	18 Tubes (1.0 to 2.0 mL)
15-350-50B	12 Tubes (4.5 to 5.0 mL)

Catalog No. 15-350-50A Supplier Thermo Scientific™ Supplier No. 51000036

Description

Freeze cells in tubes from 1 to 5 mL using this quick-freeze container designed to achieve a rate of cooling very close to -1°C/minute.

Freeze cells in tubes from 1 to 5 mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container. The simple-to-use system is designed to achieve a rate of cooling very close to -1°C/minute, the optimal rate for cell preservation. It holds 12 to 18 tubes, and a screw top lid secures samples inside in the event of accidental container tipping.

Polycarbonate containers with blue high-density polyethylene closure. Gray high-density polyethylene vial holder, foam insert.

• Holds 12 to 18 tubes and has profile height of 86 or 151mm (3-7/16 to 5-5/16 in.), which saves precious freezer space during the cooling process
• Screw top lid secures samples inside in the event of accidental container tipping
• Eliminates unreliable, improvised foam container devices
• Easy to use—requires only 100% isopropyl alcohol and a mechanical freezer
• No contact with the alcohol: no wicking, no label removal and no compromise of tube hand printing

Ordering Information:
Containers have 4.75 in. diameter (120mm) closure

Includes:
Requires:
Isopropyl alcohol and a mechanical freezer

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Specifications

• Height (Metric): 151 mm
• Diameter (Metric): 117 mm
• Material: Polycarbonate
• Type: Freezing Container
• Color: Clear, Blue Lid
• Capacity (Metric): 3.6 mL
• Holds: 12 Tubes (3.6 mL)
• Temperature Range: -1°C

Frequently Asked Questions (FAQs)

At what temperature should my Mr. Frosty controlled rate cell freezing container be when I start freezing cells?

For best cooling rate performance, the entire system should be at room temperature just prior to freezing.

Should I retain or discard the foam insert in my Mr. Frosty controlled rate cell freezing container?

The white foam insert serves no purpose other than to make the printed instructions on the jar easier to read. Its presence or absence will not affect the cooling rate, and so it can be discarded.

Why is it recommended that I change the isopropanol in my Mr. Frosty controlled rate cell freezing container after every 5 uses?

The Mr. Frosty system depends on the specific heat capacity of alcohol in order to provide the optimum cooling rate of 1 degree Celsius per minute. After several cooling and warming cycles, enough vapor condensation has taken place that much of the alcohol in the reservoir has been replaced by water, which affects the heat capacity of the assembled unit such that the cooling rate may be affected.