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Innovative Research Inc Ms anti-KLH IgG Kit
Supplier: Innovative Research Inc IMSAKLHIGGKT
Mouse Anti Keyhole Limpet Hemocyanin (anti-KLH) IgG ELISA Kit - The assay uses KLH for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-mouse IgG antibodies for detection via ELISA.Strip well format. Reagents for up to 96 tests. The assay uses KLH for solid phase (microtiter wells) immobilization and HRP conjugated anti-mouse IgG antibodies for detection. Test serum or plasma samples are diluted and incubated in the microtiter wells which are subsequently washed, and HRP conjugate is added and incubated. Anti-KLH IgG molecules are thus sandwiched between immobilized KLH and the detection antibody conjugate.Once washed to remove unbound HRP- labeled antibodies, TMB Reagent is added and incubated. Blue color development is stopped by Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of anti-KLH IgG is proportional to the optical density of the test sample.
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