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Innovative Research Inc Ms EPCR Kit
Supplier: Innovative Research Inc IMSPROCRKT
Mouse Endothelial Protein C Receptor (EPCR) ELISA Kit - This kit is for quantitative detection of activated mouse EPCR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. A monoclonal antibody from rat specific for EPCR has been precoated onto 96-well plates. Standards(Expression system for standard NSO, Immunogen sequence L18-S214) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPCR amount of sample captured in plate.
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