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Innovative Research Inc Ms Protease Nexin 1 Kit
Supplier: Innovative Research Inc IMSPN1KT
Mouse Protease Nexin 1 ELISA Kit - This kit is for quantitative detection of mouse SerpinE2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. Strip well format. Reagents for up to 96 tests. A monoclonal antibody from rat specific for SerpinE2 has been precoated onto 96-well plates. Standards(Expression system for standard NSO, Immunogen sequence S20-P397) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SerpinE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
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