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Innovative Research Inc Ms Ttl Fctr II Prthrmbn Kit
Supplier: Innovative Research Inc IMSFIIKTT
Mouse Total Factor II (Prothrombin) ELISA Kit - This kit is for the quantitative determination of total prothrombin in mouse plasma, serum, tissue extracts, and cell culture media. Thrombin and thrombin-antithrombin complex will be detected. Mouse Factor II (Prothrombin) ELISA Kit that is specific for prothrombin and will not detect thrombin or TAT complex. For RUO. Strip well format - up to 96 tests. Mouse prothrombin will bind to the capture antibody. Thrombin and thrombin-antithrombin complex will not react with the antibody coated. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color is proportional to the concentration.
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