Abnova™ MYC CISH Probe

MYC CISH Probe is designed for the qualitative detection of human MYC gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0021

Catalog No. 89-335-568

Description

The probe is intended to be used in combination with the CISH Implementation Kit 1, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYC gene region).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYC gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYC gene locus or polysomy of chromosome 8, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-P
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• Includes: Reagent Provided: 1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 8q24.21 (chr8:128,596,776-128,887,929) harboring the MYC gene 2. Formamide based hybridization buffer