Abnova™ MYCN CISH Probe

Catalog No.	For Use With (Application)
89-335-569	CISH-P

Catalog No. 89-335-569

Supplier: Abnova™ CG0022

Description

MYCN CISH Probe is designed for the qualitative detection human MYCN gene in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYCN gene).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYCN gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYCN gene locus or aneuploidy of chromosome 2, multiple copies of the brown signal or brown signal clusters will be observed.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P
• Includes: Reagent Provided:
  
  Digoxigenin-labeled oligonucleotides targeting sequences mapping in 2p24.3* (chr2:15,846,046-16,213,717) harboring the MYCN gene.
  2. Formamide based hybridization buffer.
  *According to Human Genome Assembly GRCh37/hg19