Abnova™ MYCN CISH Probe

MYCN CISH Probe is designed for the qualitative detection human MYCN gene in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CG0022

Catalog No. 89-335-569

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYCN gene).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYCN gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYCN gene locus or aneuploidy of chromosome 2, multiple copies of the brown signal or brown signal clusters will be observed.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P
• Includes: Reagent Provided:
  
  Digoxigenin-labeled oligonucleotides targeting sequences mapping in 2p24.3* (chr2:15,846,046-16,213,717) harboring the MYCN gene.
  2. Formamide based hybridization buffer.
  *According to Human Genome Assembly GRCh37/hg19