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ECM Bioscience Myosin IIA Heavy Chain (Ser-1943), phospho-specific Rabbit Polyclonal Antibody, ECM Biosciences

Supplier:  ECM Bioscience MP3831

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This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts. The immunogen was Phospho-NMHC-IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC. Accession #P35579. Applications = WB, E, ICC; Species Reactivity = Hu, Rt, Ms, Ck; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Other Names = NMHC-IIA, MYH9

Catalog No. 50-172-0820


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