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Bachem N-(4-Methoxyphenylazoformyl)-Arg-OH · HCl | 442158-31-8 | 25mg
Supplier: Bachem 4041524.0025
N-(4-Methoxyphenylazoformyl)-Arg-OH · HCl, 442158-31-8, C14H20N6O4 · HCl, Mr 372.81, 25mg. Aaf-Arg-OH. AAFR, substrate for carboxypeptidase B type enzymes. Enzymic removal of the anisylazoformyl (Aaf) moiety is followed by a decrease in substrate absorption at 350 nm (lmax ≈ 350 nm, e ≈ 19000 M-1 cm-1). The kcat/Km value for hydrolysis by porcine carboxypeptidase B at 25 °C, pH 7.9 (0.05 M Tris buffer) was 1.0 x 106 s-1 M-1. AAFR has been used to measure the TAFI (thrombin activatable fibrinolysis inhibitor, carboxypeptidase U) activation by thrombin and thrombin-thrombomodulin.
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