The Human N-myc-interactor (NMI) ELISA Kit is designed to quantitatively measure NMI levels in serum plasma tissue homogenates and cell lysates using a sandwich assay format With a detection range of 23 5 pg/mL to 1500 pg/mL and a sensitivity of 5 8 pg/mL this kit provides precise and reliable quantification of NMI across diverse biological samples The assay requires a sample volume of 50-100 L and features an optimized protocol with an incubation time of 1-5 hours balancing speed and accuracy Detection is performed at 450 nm ensuring compatibility with standard microplate readers This kit is suitable for researchers studying NMI s role in cellular processes offering robust performance for both low-concentration samples and high-throughput applications in experimental settings