Thermo Scientific N-PER Neuronal Protein Extraction Reagent
Collect proteins from all cellular compartments of neuronal tissue and primary cultured neurons with this proprietary, neuronal-specific cell lysis reagent.
Manufacturer: Thermo Scientific 87792
Thermo Scientific N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons.Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90µg of protein per mg of brain tissue or 300µg of total protein from 106 primary neuronal cells.
Optimized efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells
Gentle preserves protein function without compromising yield
Versatile can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors
Compatible extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods
Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis
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|Upon receipt store product at 4°C.|
|Detergent Solution, Cell Lysis Buffer|
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