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Thermo Scientific™ N-PER™ Neuronal Protein Extraction Reagent Available on GSA/VA Contract for Federal Government customers only.

Collect proteins from all cellular compartments of neuronal tissue and primary cultured neurons with this proprietary, neuronal-specific cell lysis reagent.

Manufacturer:  Thermo Scientific™ 87792

Catalog No. PI87792


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Description

Description

Thermo Scientific™ N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons.

Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90µg of protein per mg of brain tissue or 300µg of total protein from 106 primary neuronal cells.

Highlights:

  • Optimized – efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells

  • Gentle – preserves protein function without compromising yield

  • Versatile – can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors

  • Compatible – extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods

Recommended for:

Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis

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Specifications

Specifications

Upon receipt store product at 4°C.
Liquid
NE-PER™
Detergent Solution, Cell Lysis Buffer
SDS
Documents
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