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Cell Biolabs Inc NAD+/NADH Assay Kit (Fluorometric), 100 assays
Supplier: Cell Biolabs Inc MET5030
Cell Biolabs’ NAD+/NADH Assay Kit (Fluorometric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well fluorescence plate reader. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 0.8 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.
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