Thermo Scientific NanoDrop™ Reagents

Catalog No.	Type
CF1	CF-1 Calibration Check Fluid
CF8	CF-8 Calibration Check Kit
PR1KIT	ND PR-1 Reconditioning Kit
13-400-515	PV-1 Performance Verification

Catalog No. CF1 Supplier Thermo Scientific Supplier No. CHEMCF1

Description

Certified standards for verifying instrument performance to factory specifications are available separately.

For use with Thermo Scientific NanoDrop Spectrophotometers.

• Ten times more concentrated compared to other K₂Cr₂O₇ calibration solutions
• Packaged in 0.5mL snap-top glass ampules
• Intended for single use only

Specifications

• Type: CF-1 Calibration Check Fluid
• For Use With (Equipment): NanoDrop 8000, NanoDrop Lite, NanoDrop 2000, NanoDrop 2000c spectrophotometers
• Packaging: 0.5 mL snap-top glass ampules

Frequently Asked Questions (FAQs)

How do I check the accuracy of my NanoDrop 2000/2000c Spectrophotometer?

Our NanoDrop CF-1 Calibration Fluid should be used with the Calibration Check module of the instrument software. CF-1 is prepared from the NIST potassium dichromate standard SRM935 in acidified reagent grade water (Certificate of Analysis available from NanoDrop products).

How often do I need to check the calibration of my NanoDrop Lite Spectrophotometer?

We recommend confirming that your NanoDrop Lite Spectrophotometer is working within specifications every 6 months. The NanoDrop CF-1 Calibration Fluid is required to run the calibration check procedure. CF-1 is available from Thermo Fisher website (Cat. No. CHEM-CF-1)

How do I check the accuracy of my NanoDrop Lite Spectrophotometer?

The accuracy of your NanoDrop Lite Spectrophotometer can be checked by using the NanoDrop CF-1 Calibration Fluid to perform a calibration check. CF-1 is prepared from the NIST potassium dichromate standard SRM935 in acidified reagent grade water.

My NanoDrop 8000 Spectrophotometer failed calibration check. What should I do?

Please review the following steps to take when/if your NanoDrop 8000 Spectrophotometer fails a calibration check:
- Clean the pedestal with the NanoDrop PR-1 Reconditioning Kit
- Repeat calibration check using a FRESH vial of NanoDrop CF-1 Calibration Fluid and FRESH blanking solution
- If instrument continues to be out of specification, Contact Technical Support

How do I check the calibration of my NanoDrop 8000 Spectrophotometer? Do I need to calibrate it?

You can easily check the calibration of your NanoDrop 8000 Spectrophotometer by using the NanoDrop CF-8 Calibration Check Kit, which contains two CF-1 Ampules and two 8-well PCR tube strips. The CF-8 kit is available from the Thermo Fisher website (Cat. No. CHEM-CF-8).
If re-calibration is ever needed, it is performed by a trained service technician. Due to the precise nature of the NanoDrop instrument design, these spectrophotometers rarely require re-calibration.

My NanoDrop 2000/2000c Spectrophotometer failed the calibration check. What should I do?

Please review the following steps to take when/if your NanoDrop 2000/2000c fails a calibration check:
- Clean the pedestal with the NanoDrop PR-1 Reconditioning Kit
- Repeat calibration check using a FRESH vial of NanoDrop CF-1 Calibration Fluid and FRESH blanking solution
More detailed instructions can be found on the Calibration Check Procedure document: https://assets.thermofisher.com/TFS-Assets/CAD/Product-Bulletins/NanoDrop-2000-2000c-1000-Calibration-Check-procedure.pdf
If your NanoDrop 2000/2000c continues to be out of specification, Contact Technical Support.

How often should I check the calibration of my NanoDrop 2000/2000c Spectrophotometer?

We recommend you check the calibration of your NanoDrop 2000/2000c every 6 months using the NanoDrop CF-1 Calibration Fluid.

How do I check the calibration of my NanoDrop 2000/2000c Spectrophotometer? Do I need to calibrate it?

You can easily check the calibration of your NanoDrop 2000/2000c Spectrophotometer by using NanoDrop CF-1 Calibration Fluid. CF-1 is specifically designed for NanoDrop spectrophotometers and is available from the Thermo Fisher website (Cat. No. CHEM-CF-1).
If re-calibration is ever needed, it is performed by a trained service technician. Due to the precise nature of the NanoDrop instrument design, these spectrophotometers seldom require re-calibration.

When using a NanoDrop spectrophotometer, what is the difference between controls versus standards?

NanoDrop CF-1 Calibration Fluid is the only photometric standard available to assess the accuracy of the NanoDrop spectrophotometer pedestal. If the control sample shows unexpected results the user should confirm the calibration of the instrument with the CF-1 fluid.
Some control examples include:
- Nucleic Acids: Fermentas GeneRuler Express DNA Ladder 100-5000 bp (Cat. No. SM1551)
- Protein: Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Cat. No. 23209)

How can I check the accuracy of my NanoDrop spectrophotometer?

The accuracy of NanoDrop spectrophotometers can be assessed by using NanoDrop CF-1 Calibration Fluid in conjunction with the calibration check application in the NanoDrop software.