Learn More
Thermo Scientific™ National Scientific UV Detector Performance Validation Kit
Description
Includes
Kit contains eight 30mL bottles of potassium chromate solution with concentrations from 0.1 to 0.20mg/mL (concentrations certified to four significant figures). To calibrate, fill detector cell with solution, then read absorbance. Microsoft™ Excel™ and Lotus templates (included) calculate slope, intercept, and correlation coefficient, as well as plot absorbance vs. concentration curve. Wavelength accuracy. Complete kit includes eight absorbance solutions (30mL each), one wavelength calibration solution (30mL), one absorbance reference solution (60mL), Microsoft Excel and Lotus template disk, syringes and HPLC detector connection kit, and Certificate of Analysis.
Tests detector precision, accuracy, and linearity in less than 30 minutes
Verifies performance of UV-visible detectors in UV spectrophotometers, HPLC, and CE detectors. Confirms detector wavelength settings and linear response when validating new methods—ideal for methods using extinction coefficients or factors. Quick, three-step method measures absorbance linearity, wavelength accuracy, and wavelength precision. Absorbance linearity. Kit uses holmium oxide solution as wavelength reference standard. Calibrates at 241, 278, 287, 361, 451, and 537nm. To check wavelength accuracy, fill cell with holmium oxide solution and measure absorbance ±5nm from target wavelength. Wavelength error is the difference between observed wavelength at maximum absorbance (λmax) and target wavelength.Wavelength precision. To determine wavelength precision, calculate wavelength accuracy, then set detector wavelength at least 10nm from target wavelength. Reset wavelength to target wavelength and record absorbance. Wavelength precision equals the relative standard deviation of five measurements.
Order Info
Replacement solutions are available; contact your Customer Service Representative.
Specifications
Specifications
| Type | UV Detector Performance Validation Kit |
| For Use With (Equipment) | UV-Visible Detector |
| For Use With (Application) | Verifies Performance of UV-Visible Detectors in UV Spectrophotometers, HPLC and CE Detectors |
| Includes | Eight 30 mL absorbance solutions, one 30 mL wavelength calibration solution, one 60 mL absorbance reference solution, Microsoft excel and lotus template disk, syringes and HPLC detector connection kit and certificate of analysis |
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.