Thermo Scientific™ National Scientific UV Detector Performance Validation Kit

Supplier:  Thermo Scientific™ CK09001S

Catalog No. 13-375-61

Includes: Kit contains eight 30mL bottles of potassium chromate solution with concentrations from 0.1 to 0.20mg/mL (concentrations certified to four significant figures). To calibrate, fill detector cell with solution, then read absorbance. Microsoft™ Excel™ and Lotus templates (included) calculate slope, intercept, and correlation coefficient, as well as plot absorbance vs. concentration curve. Wavelength accuracy. Complete kit includes eight absorbance solutions (30mL each), one wavelength calibration solution (30mL), one absorbance reference solution (60mL), Microsoft Excel and Lotus template disk, syringes and HPLC detector connection kit, and Certificate of Analysis.

Description

Tests detector precision, accuracy, and linearity in less than 30 minutes

Verifies performance of UV-visible detectors in UV spectrophotometers, HPLC, and CE detectors. Confirms detector wavelength settings and linear response when validating new methods—ideal for methods using extinction coefficients or factors. Quick, three-step method measures absorbance linearity, wavelength accuracy, and wavelength precision. Absorbance linearity. Kit uses holmium oxide solution as wavelength reference standard. Calibrates at 241, 278, 287, 361, 451, and 537nm. To check wavelength accuracy, fill cell with holmium oxide solution and measure absorbance ±5nm from target wavelength. Wavelength error is the difference between observed wavelength at maximum absorbance (λ_max) and target wavelength.Wavelength precision. To determine wavelength precision, calculate wavelength accuracy, then set detector wavelength at least 10nm from target wavelength. Reset wavelength to target wavelength and record absorbance. Wavelength precision equals the relative standard deviation of five measurements.

Order Info

Replacement solutions are available; contact your Customer Service Representative.

Specifications

• Type: UV Detector Performance Validation Kit
• For Use With (Equipment): UV-Visible Detector
• For Use With (Application): Verifies Performance of UV-Visible Detectors in UV Spectrophotometers, HPLC and CE Detectors
• Includes: Eight 30 mL absorbance solutions, one 30 mL wavelength calibration solution, one 60 mL absorbance reference solution, Microsoft excel and lotus template disk, syringes and HPLC detector connection kit and certificate of analysis