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New England Biolabs, Inc. NEBNext UltraShear - 96 rxns
Supplier: New England Biolabs, Inc. M7634L
Enzymatic fragmentation of DNA as part of the library prep workflow provides many advantages compared to mechanical shearing. However, specialized fragmentation reagents are required for enzymatic shearing of challenging samples such as FFPE DNA, and in order to maintain methylation marks on samples for methylome analysis. NEBNext UltraShear is a mix of enzymes that has been designed and optimized to fragment these sample types upstream of library preparation. This improves library yields and diversity, and allows retention of methylation marks. NEBNext UltraShear fragments DNA in a time-dependent manner and the same protocol is followed for all input amounts (5 ng – 250 ng).
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