Invitrogen™ Human NEK4, GST Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length

NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It is proposed to act exclusively upon threonine residues and is present in most primary carcinomas. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Specifications

• Accession Number: P51957
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris HCl with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 6787
• Molecular Weight (g/mol): 122 kDa
• Name: Human NEK4, GST Tag
• Quantity: 10 μg
• Gene Alias: Nek4; Never in mitosis A-related kinase 4; NIMA (never in mitosis gene a)-related expressed kinase 4; NIMA (never in mitosis gene a)-related kinase 4; NIMA related kinase 4; NIMA-related kinase 4; nimA-related protein kinase 4; NRK2; pp12301; serine/threonine kinase 2; serine/threonine protein kinase-2; serine/threonine-protein kinase 2; Serine/threonine-protein kinase Nek4; serine/threonine-protein kinase Nek4 variant 2; serine/threonine-protein kinase NRK2; STK2
• Common Name: NEK4
• KinaseFamily: NEK Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Kinase Group: Ser/Thr Kinases: Other
• Shipping Condition: Dry Ice
• System Compatibility: Zin-LYTE™
• Target Entry: NEK4
• Target Gene: NIMA (never in mitosis gene a)-related expressed kinase 4
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction