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New England Biolabs, Inc. NEBNext Q5U™ Master Mix – 250 reactions

Supplier: New England Biolabs, Inc. M0597L

NEBNext Q5U Master Mix is optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. The polymerase component of the master mix, Q5U, is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3´->5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. The NEBNext Q5U Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.
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