Meridian Life Science NGS Library Quantification, 1ML

Supplier:  Meridian Life Science MDX0391

Accurate quantification of the number of amplifiable library molecules loading into a flow cell is one of the most critical steps in the next-generation sequencing (NGS) workflow in obtaining high-quality read data. Loading insufficient library DNA will result in low cluster density and reduced sequencing yield. An overabundance of library DNA may increase cluster density and result in poor quality data. Standard methods of NGS library quantification, by electrophoresis or spectrophotometry, have low sensitivity, are non-specific for adapter-bound DNA and typically require a large amount of library sample for analysis. With its greater sensitivity and broad dynamic range, Meridian’s NGS Library Quantification Kit is an optimized, robust qPCR kit containing pre-diluted standards to minimize pipetting errors and a pre-qualified P5 and P7 Illumina adaptor sequence primer mix to ensure reproducible and precise qPCR results and an optimized buffer for dilution of NGS library samples.

Catalog No. 50-008-56340