Used in the detection and assay of peptides amino acids amines and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines Reaction with sarcosine or proline gives azomethine ylides Application It can be used for the detection of peptides amino acids amino acids and amino sugars and can produce strong fluorescent ternary compounds with aldehydes and primary amines Reacts with sarcosine or proline to form methylimine Ylide Ninhydrin (ninhydrin hydrate) can be used as a reagent for the determination of amino acids and peptides by photometry and colorimetry It can also be used to prepare ninhydrin reagents by mixing ninhydrin with hydroninhydrin (reduced form of ninhydrin) in dimethyl sulfoxide This reagent can be used for the manual ninhydrin method to quantify amino acids It can be used to detect free amino groups in amino acids peptides and proteins