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Nitrotetrazolium Blue Chloride, Sigma-Aldrich

Catalog No. 01258917 Shop All MilliporeSigma Supelco Products
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250 mg
500 mg
1 g
5 g

Catalog No. 01-258-917

Supplier: MilliporeSigma Supelco N68761G

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Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Nitrotetrazolium Blue chloride
CAS 298-83-9
Molecular Weight (g/mol) 817.64 g/mol
Percent Purity ≥90%
Color Yellow
Quantity 1 g
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