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Description
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.
Specifications
Specifications
| Chemical Name or Material | Nitrotetrazolium Blue chloride |
| CAS | 298-83-9 |
| Molecular Weight (g/mol) | 817.64 g/mol |
| Percent Purity | ≥90% |
| Color | Yellow |
| Quantity | 1 g |
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