Invitrogen™ NovaFluor™ Blue 725 Conjugation Kit, 100 μg

Description

Get the most out of your panel design by conjugating antibodies to NovaFluors: dyes specifically designed for spectral hygiene to have narrow emission spectra and minimal cross-laser excitation.

NovaFluor Conjugation Kits provide a simple workflow to conjugate a blue, yellow-green or red excitable fluorophore to your antibody of choice in just 3 easy steps. The fluorescence-by-design Phiton platform enables the addition of parameters to your current panels in discovery research and resolves stained populations with near-zero cross excitation, with a lack of emission in the violet channels that plague other fluorophores.

NovaFluor Conjugation Kit provides features including:

• A clean and bright antibody label to increase the number of parameters on conventional and spectral flow cytometers
• Resolve populations with limited/near-zero cross excitation and the lowest spread performance of any blue, yellow-green or red excited fluorescent labels
• Simple 3-step protocol, with 60 sec. of hands-on time for conjugating 10–100 μg of antibody per label
• Up to 70% less excitation when compared to PE tandems, resulting in decreased spillover and higher resolution

3-step conjugation method

• Attach NovaFluor Linker to antibody of choice
• Remove excess NovaFluor Linker from DNA-conjugate antibody
• Anneal NovaFluor to oligo-conjugated antibody

Specifications

• For Use With (Application): Flow Cytometry
• Quantity: 100 μg
• Product Type: Conjugation Kit
• Label or Dye: NovaFluor™ Blue 725
• Product Line: NovaFluor™
• Emission: 725 nm

For Research Use Only. Not for use in diagnostic procedures.