MilliporeSigma™ Novagen™ BCA Protein Assay Kit

Description

Determines protein concentration in standard assay or microassay configuration.

EMD Millipore Novagen™ BCA Protein Assay Kit is based on biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in alkaline solution.

• Sufficient to complete 500 standard-size reactions or or 2500 microscale reactions
• Used to determine protein concentrationin the range of 20-2000 μg/mL
• Robust, can be performed in presence of many compounds
• BSA standard (2 mg/mL) is provided for convenient and reliable preparation of standard curves

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Specifications

• Sufficient For: 500 standard-size reactions (50 μL protein sample plus 1 mL reagent) or 2500 microscale reactions (25 μL protein sample plus 200 μL reagent)
• Kit Contents: 500 mL BCA Solution, 15 mL 4% Cupric Sulfate, 3 x 1 mL BSA Standard, 2 mg/mL, Poly(dI-dC) solution, Sonicated salmon sperm DNA
• Quantitation Range: 20 to 2000 μg/mL
• Quantity: 1 Kit
• Storage Requirements: Store at room temperature