MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium - 15N 13C
Used to optimize cell growth and protein expression before preparing isotopically labeled proteins for NMR spectroscopy. MilliporeSigma™; Novagen™ Overnight Express™ Autoinduction NMR Optimization Medium is based on the differential metabolism of carbon sources to promote growth to high cell density.
Manufacturer: MilliporeSigma™ 717893
- Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
- Includes four concentrated sterile solutions:
- OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
- OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
- OnEx Solution 3 provides magnesium for maximal cell density
- OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels
|Multiple storage requirements. Ok to freeze.|
|Autoinduction NMR Medium - 15N 13C|
|1 L Kit|
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