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MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium - 15N 13C

Catalog No. 717893 Shop All MilliporeSigma Products
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71-789-3 1 L Kit
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Catalog No. 71-789-3 Supplier MilliporeSigma™ Supplier No. 717893
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Description

Used to optimize cell growth and protein expression before preparing isotopically labeled proteins for NMR spectroscopy. MilliporeSigma™; Novagen™ Overnight Express™ Autoinduction NMR Optimization Medium is based on the differential metabolism of carbon sources to promote growth to high cell density.

  • Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
  • Includes four concentrated sterile solutions:
  • OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
  • OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
  • OnEx Solution 3 provides magnesium for maximal cell density
  • OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels
TRUSTED_SUSTAINABILITY

Specifications

Quantity 1 L Kit
Content And Storage Multiple storage requirements. Ok to freeze.
Product Type Autoinduction NMR Medium - 15N 13C
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