Invitrogen™ Human Amyloid beta 42 ELISA Kit

Description

Includes

Pre-coated plate, standard, detector antibody, anti-IgG-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Amyloid Beta 42 (Hu Abeta 42) ELISA quantitates Hu Abeta 42 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 42. Principle of the method The Human Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P05067
• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: <10 pg/mL
• Concentration: 15.6 to 1000 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cerebrospinal Fluid, Supernatant, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 351
• Gene Symbol: AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
• Incubation Time: 4 hr.
• Interassay CV: 4.3%
• Intraassay CV: 3.6
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, Anti-IgG HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: Aβ 42
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cerebrospinal Fluid 50 μL, Supernatant 50 μL, Tissue Homogenate 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Amyloid beta 42
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs

For Research Use Only. Not for use in diagnostic procedures.