Invitrogen™ Human c-Met (Soluble) ELISA Kit

Description

ELISA

The Human c-Met (Soluble) ELISA quantitates Hu c-Met in human serum, plasma and cell culture supernatant. The assay will exclusively recognize both natural and recombinant Hu c-Met. Principle of the method The Human c-Met solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

Specifications

• Accession Number: P08581
• Assay Range: 0.78 to 50 ng/mL
• Assay Sensitivity: <0.5 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4233
• Gene Symbol: AUTS9,DFNB97,HGFR,RCCP2,c-Met
• Incubation Time: 4 hr.
• Interassay CV: 8.1%
• Intraassay CV: 6.1
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: c-Met (soluble)
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: c-Met
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs