Invitrogen™ Human CRP ELISA Kit

Description

ELISA

The Human C-Reactive Protein(Hu CRP) ELISA quantitates Hu CRP in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu CRP. Principle of the method The Human CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.

Specifications

• Accession Number: P02741
• Assay Range: 18.75 to 1200 pg/mL
• Assay Sensitivity: <10 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1401
• Gene Symbol: PTX1
• Incubation Time: 4 hr.
• Interassay CV: 9.7%
• Intraassay CV: 7
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: CRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 5 μL, Serum 5 μL
• Storage Requirements: 2°C to 8°C
• Target: CRP
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs