Invitrogen™ Human Insulin ELISA Kit

Description

ELISA

The Human Insulin ELISA Kit quantifies human insulin in serum. The assay will recognize both the natural and recombinant insulin. Principle of the method The Human insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

Specifications

• Accession Number: P01308
• Assay Range: 5.1 to 250 μlU/mL
• Assay Sensitivity: 0.17 μIU/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3630
• Gene Symbol: IDDM,IDDM1,IDDM2,ILPR,IRDN,MODY10
• Incubation Time: 0.75 hr.
• Interassay CV: 8.5%
• Intraassay CV: 5.4
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Marker Gene: Insulin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Insulin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 45 mins