Invitrogen™ Human Osteocalcin ELISA Kit

Description

ELISA

The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum and plasma,. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin. Principle of the method The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Specifications

• Accession Number: P02818
• Assay Range: 1.56 to 75 ng/mL
• Assay Sensitivity: 0.08 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 632
• Gene Symbol: BGP,OC,OCN
• Incubation Time: 2.5 hr.
• Interassay CV: 4.6%
• Intraassay CV: 3.9
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Marker Gene: Osteocalcin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 25 μL, Serum 25 μL
• Storage Requirements: 2°C to 8°C
• Target: Osteocalcin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins