Invitrogen™ Human Aggrecan (PG) ELISA Kit

Description

ELISA

The Human Aggrecan (PG) ELISA quantitates Hu Aggrecan (PG) in humansynovial fluid, serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Aggrecan (PG). Principle of the method The Human Aggrecan (PG) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second enzyme-conjugated (detector) antibody, binding to the target on a different epitope from the capture antibody. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

Specifications

• Accession Number: P16112
• Assay Range: 10 to 250 ng/mL
• Assay Sensitivity: 0.9 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum, Supernatant, Synovial Fluid
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 176
• Gene Symbol: AGC1,AGCAN,CSPG1,CSPGCP,MSK16,SEDK
• Incubation Time: 3.25 hr.
• Interassay CV: 5.6%
• Intraassay CV: 2.1
• Kit Contents: Pre-coated 96 well plate, Standard, Sample diluent, Incubation Buffer, HRP conjugated Detection Antibody, Conjugate Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Marker Gene: PG
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 20 μL, Supernatant 10 μL, Synovial Fluid 25 μL
• Storage Requirements: 2°C to 8°C
• Target: Aggrecan
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 15 mins