Invitrogen™ Human IL-10 ELISA Kit

Description

Includes

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Interlukin 10 (IL-10) ELISA quantitates Hu IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10. Principle of the method The Human IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Order Info

Shipping Condition: Wet ice

Specifications

• Accession Number: P22301
• Assay Range: 7.8 to 500 pg/mL
• Assay Sensitivity: <1 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3586
• Gene Symbol: CSIF,GVHDS,IL-10 A,TGIF,IL10
• Interassay CV: 3.7%
• Intraassay CV: 3.5
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Precision: <1 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-10
• Test Time: 1 hrs 20 mins
• Total Assay Time: 5 hrs

For Research Use Only. Not for use in diagnostic procedures.