Invitrogen™ Mouse IL-13 ELISA Kit

Catalog No.	Quantity
KMC2221	96 Tests

Catalog No. KMC2221 Supplier Invitrogen™ Supplier No. KMC2221

Description

ELISA

The Mouse Interleukin 13 (Ms IL-13) ELISA quantitates Ms IL-13 in mouse serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-13. Some cross-reactivity is observed in human and rat samples. Principle of the method The Mouse IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

Specifications

• Accession Number: P20109
• Assay Range: 3.9 to 250 pg/mL
• Assay Sensitivity: <2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16163
• Gene Symbol: IL-13
• Interassay CV: 8%
• Intraassay CV: 6.7
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Precision: <2 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-13
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs