Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Supplier:  Invitrogen™ R90115

Catalog No. R90115

Includes: The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺

Description

• Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
• Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
• One-step purification can be performed under both native and denaturing conditions
• Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
• Guaranteed stable for 6 months when properly stored at 4°C

Specifications

• Stationary Phase: Ni-NTA
• Quantity: 25 mL
• Type: Agarose

• Column Type: Affinity Column
• Form: Suspension
• Content And Storage: The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.

For Research Use Only. Not for use in diagnostic procedures.