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Invitrogen™ NuPAGE™ Large Protein Blotting Kit
Description
The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards offer a time-saving load-and-go format. The HiMark Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460kDa in NuPAGE Tris-Acetate Gel/SDS buffer system. It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins. The HiMark Unstained Standard, along with the NuPAGE Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems. Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. Several NuPAGE Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for high-resolution large protein separation and analysis. Each kit includes two boxes of NuPAGE 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark Standard.
- Gel Percentage: 3-8 %
- Gel Size: Mini (8 x 8 cm)
- Well Format: 1D Well
1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting
Specifications
Specifications
| Content And Storage | See product manual for kit contents and component storage conditions. Components of each kit are also available individually. |
| For Use With (Equipment) | XCell SureLock Mini-Cell |
| Gel Percentage | 3 to 8% |
| Gel Size | Mini |
| Gel Type | Tris-Acetate |
| Product Type | Protein Blotting Kit |
| Sample Loading Volume | 25 μL |
| Separation Range | 40 to 500 kDa |
| Stain Type | Unstained |
| Wells | 10-well |
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For Research Use Only. Not for use in diagnostic procedures.
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